Gene description for Cyb5a
Gene name cytochrome b5 type A (microsomal)
Gene symbol Cyb5a
Other names/aliases Cyb5
Species Rattus norvegicus
 Database cross references - Cyb5a
ExoCarta ExoCarta_64001
Vesiclepedia VP_64001
Entrez Gene 64001
UniProt P00173  
 Cyb5a identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Cyb5a
Molecular Function
    electron transfer activity GO:0009055 TAS
    enzyme binding GO:0019899 ISO
    heme binding GO:0020037 IBA
    heme binding GO:0020037 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    response to cadmium ion GO:0046686 IEP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 ISO
    membrane GO:0016020 ISO
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Cyb5a in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Cyb5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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