Gene description for Hspa2
Gene name heat shock protein 2
Gene symbol Hspa2
Other names/aliases Hspt70
Hst70
Species Rattus norvegicus
 Database cross references - Hspa2
ExoCarta ExoCarta_60460
Vesiclepedia VP_60460
Entrez Gene 60460
UniProt P14659  
 Hspa2 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Hspa2
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    enzyme binding GO:0019899 ISO
    heat shock protein binding GO:0031072 IBA
    protein folding chaperone GO:0044183 IBA
    tau protein binding GO:0048156 IPI
    unfolded protein binding GO:0051082 ISO
    protein-folding chaperone binding GO:0051087 IPI
    glycolipid binding GO:0051861 ISO
    disordered domain specific binding GO:0097718 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 ISO
    male meiotic nuclear division GO:0007140 ISO
    male meiosis I GO:0007141 ISO
    spermatogenesis GO:0007283 ISO
    spermatogenesis GO:0007283 ISS
    spermatid development GO:0007286 ISO
    response to heat GO:0009408 ISS
    response to cold GO:0009409 ISS
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 ISO
    positive regulation of ATP-dependent activity GO:0032781 ISO
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    synaptonemal complex disassembly GO:0070194 ISO
    negative regulation of inclusion body assembly GO:0090084 ISO
    positive regulation of ATPase-coupled calcium transmembrane transporter activity GO:1901896 ISO
Subcellular Localization
    synaptonemal complex GO:0000795 ISO
    male germ cell nucleus GO:0001673 ISO
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    cell surface GO:0009986 ISO
    CatSper complex GO:0036128 ISO
    CatSper complex GO:0036128 ISS
    meiotic spindle GO:0072687 ISO
    meiotic spindle GO:0072687 ISS
 Experiment description of studies that identified Hspa2 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hspa2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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