Gene description for Gde1
Gene name glycerophosphodiester phosphodiesterase 1
Gene symbol Gde1
Other names/aliases Mir16
Species Rattus norvegicus
 Database cross references - Gde1
ExoCarta ExoCarta_60418
Vesiclepedia VP_60418
Entrez Gene 60418
UniProt Q9JL55  
 Gde1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gde1
Molecular Function
    lysophospholipase activity GO:0004622 ISO
    lysophospholipase activity GO:0004622 ISS
    phosphoric diester hydrolase activity GO:0008081 IEA
    phosphoric diester hydrolase activity GO:0008081 ISO
    glycerophosphodiester phosphodiesterase activity GO:0008889 IBA
    glycerophosphodiester phosphodiesterase activity GO:0008889 ISO
    glycerophosphodiester phosphodiesterase activity GO:0008889 ISS
    metal ion binding GO:0046872 IEA
    glycerophosphoinositol glycerophosphodiesterase activity GO:0047395 ISO
    glycerophosphoinositol glycerophosphodiesterase activity GO:0047395 ISS
Biological Process
    ethanolamine metabolic process GO:0006580 IBA
    ethanolamine metabolic process GO:0006580 ISO
    ethanolamine metabolic process GO:0006580 ISS
    lipid metabolic process GO:0006629 IEA
    phospholipid metabolic process GO:0006644 IBA
    phospholipid metabolic process GO:0006644 ISO
    phospholipid metabolic process GO:0006644 ISS
    G protein-coupled receptor signaling pathway GO:0007186 NAS
    N-acylethanolamine metabolic process GO:0070291 IBA
    N-acylethanolamine metabolic process GO:0070291 ISO
    N-acylethanolamine metabolic process GO:0070291 ISO
    N-acylethanolamine metabolic process GO:0070291 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    membrane GO:0016020 ISO
    cytoplasmic vesicle membrane GO:0030659 IEA
 Experiment description of studies that identified Gde1 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gde1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here