Gene description for Ago2
Gene name argonaute RISC catalytic component 2
Gene symbol Ago2
Other names/aliases Eif2c2
Species Rattus norvegicus
 Database cross references - Ago2
ExoCarta ExoCarta_59117
Vesiclepedia VP_59117
Entrez Gene 59117
UniProt Q9QZ81  
 Ago2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Ago2
Molecular Function
    RNA 7-methylguanosine cap binding GO:0000340 IEA
    RNA 7-methylguanosine cap binding GO:0000340 ISO
    RNA 7-methylguanosine cap binding GO:0000340 ISS
    RNA polymerase II complex binding GO:0000993 IEA
    RNA polymerase II complex binding GO:0000993 ISO
    core promoter sequence-specific DNA binding GO:0001046 IEA
    core promoter sequence-specific DNA binding GO:0001046 ISO
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    double-stranded RNA binding GO:0003725 IEA
    double-stranded RNA binding GO:0003725 ISO
    single-stranded RNA binding GO:0003727 IBA
    single-stranded RNA binding GO:0003727 IEA
    single-stranded RNA binding GO:0003727 ISO
    mRNA binding GO:0003729 ISO
    RNA endonuclease activity GO:0004521 IBA
    RNA endonuclease activity GO:0004521 ISO
    protein binding GO:0005515 IPI
    hydrolase activity GO:0016787 IEA
    Hsp70 protein binding GO:0030544 IDA
    siRNA binding GO:0035197 IEA
    siRNA binding GO:0035197 ISO
    siRNA binding GO:0035197 ISO
    siRNA binding GO:0035197 ISS
    miRNA binding GO:0035198 IBA
    miRNA binding GO:0035198 IEA
    miRNA binding GO:0035198 ISO
    mRNA 3'-UTR AU-rich region binding GO:0035925 IEA
    mRNA 3'-UTR AU-rich region binding GO:0035925 ISO
    metal ion binding GO:0046872 IEA
    Hsp90 protein binding GO:0051879 IDA
    endoribonuclease activity, cleaving siRNA-paired mRNA GO:0070551 IEA
    endoribonuclease activity, cleaving siRNA-paired mRNA GO:0070551 ISO
    endoribonuclease activity, cleaving siRNA-paired mRNA GO:0070551 ISS
    endoribonuclease activity, cleaving miRNA-paired mRNA GO:0090624 IEA
    endoribonuclease activity, cleaving miRNA-paired mRNA GO:0090624 ISO
    mRNA cap binding GO:0098808 IEA
    mRNA cap binding GO:0098808 ISO
    mRNA cap binding GO:0098808 ISS
    RISC complex binding GO:1905172 IDA
Biological Process
    response to hypoxia GO:0001666 IEP
    regulation of DNA-templated transcription GO:0006355 IEA
    regulation of translation GO:0006417 IEA
    spermatogenesis GO:0007283 IEP
    post-embryonic development GO:0009791 IEA
    post-embryonic development GO:0009791 ISO
    RNA secondary structure unwinding GO:0010501 IEA
    RNA secondary structure unwinding GO:0010501 ISO
    miRNA metabolic process GO:0010586 IEA
    miRNA metabolic process GO:0010586 ISO
    positive regulation of gene expression GO:0010628 ISO
    cell differentiation GO:0030154 NAS
    siRNA processing GO:0030422 IEA
    siRNA processing GO:0030422 ISO
    regulatory ncRNA-mediated gene silencing GO:0031047 IEA
    regulatory ncRNA-mediated gene silencing GO:0031047 ISO
    regulatory ncRNA-mediated gene silencing GO:0031047 ISS
    pre-miRNA processing GO:0031054 IBA
    pre-miRNA processing GO:0031054 IEA
    pre-miRNA processing GO:0031054 ISO
    pre-miRNA processing GO:0031054 ISS
    P-body assembly GO:0033962 IEA
    P-body assembly GO:0033962 ISO
    regulatory ncRNA-mediated post-transcriptional gene silencing GO:0035194 IBA
    miRNA processing GO:0035196 ISO
    miRNA processing GO:0035196 ISO
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 IEA
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISO
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISS
    miRNA-mediated gene silencing by mRNA destabilization GO:0035279 IEA
    miRNA-mediated gene silencing by mRNA destabilization GO:0035279 ISO
    miRNA-mediated gene silencing by mRNA destabilization GO:0035279 ISS
    response to cocaine GO:0042220 IEP
    negative regulation of amyloid precursor protein biosynthetic process GO:0042985 IMP
    response to morphine GO:0043278 IEP
    regulation of mRNA stability GO:0043488 IMP
    positive regulation of translation GO:0045727 IEA
    positive regulation of translation GO:0045727 ISO
    positive regulation of angiogenesis GO:0045766 IEA
    positive regulation of angiogenesis GO:0045766 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    negative regulation of translational initiation GO:0045947 IEA
    negative regulation of translational initiation GO:0045947 ISO
    negative regulation of translational initiation GO:0045947 ISS
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 IEA
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 ISO
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 ISS
    RISC complex assembly GO:0070922 IEA
    RISC complex assembly GO:0070922 ISO
    cellular response to calcium ion GO:0071277 IEP
    regulation of synapse maturation GO:0090128 IEA
    regulation of synapse maturation GO:0090128 ISO
    siRNA-mediated gene silencing by mRNA destabilization GO:0090625 IEA
    siRNA-mediated gene silencing by mRNA destabilization GO:0090625 ISO
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 IEA
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 ISO
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 ISS
    positive regulation of post-transcriptional gene silencing by RNA GO:1900370 IMP
    positive regulation of trophoblast cell migration GO:1901165 IEA
    positive regulation of trophoblast cell migration GO:1901165 ISO
Subcellular Localization
    P-body GO:0000932 IEA
    P-body GO:0000932 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    cytoplasmic stress granule GO:0010494 IDA
    membrane GO:0016020 IDA
    RISC complex GO:0016442 IBA
    RISC complex GO:0016442 IDA
    RISC complex GO:0016442 IEA
    RISC complex GO:0016442 ISO
    RISC complex GO:0016442 ISS
    dendrite GO:0030425 IDA
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    chromatoid body GO:0033391 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IBA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    P granule GO:0043186 IDA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    RISC-loading complex GO:0070578 IEA
    RISC-loading complex GO:0070578 ISO
    RISC-loading complex GO:0070578 ISS
    pi-body GO:0071546 IDA
    postsynapse GO:0098794 IEA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
    ribonucleoprotein complex GO:1990904 IDA
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Ago2 in sEVs
1
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ago2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ago2 is involved
No pathways found





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