Gene description for Carm1
Gene name coactivator-associated arginine methyltransferase 1
Gene symbol Carm1
Other names/aliases Prmt4
Species Mus musculus
 Database cross references - Carm1
ExoCarta ExoCarta_59035
Vesiclepedia VP_59035
Entrez Gene 59035
UniProt Q9WVG6  
 Carm1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Carm1
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IMP
    transcription coactivator activity GO:0003713 IDA
    transcription coactivator activity GO:0003713 IMP
    protein binding GO:0005515 IPI
    protein methyltransferase activity GO:0008276 IDA
    protein methyltransferase activity GO:0008276 ISO
    histone arginine N-methyltransferase activity GO:0008469 ISO
    protein-arginine N-methyltransferase activity GO:0016274 IDA
    protein-arginine N-methyltransferase activity GO:0016274 ISO
    nuclear receptor coactivator activity GO:0030374 IDA
    protein-arginine omega-N asymmetric methyltransferase activity GO:0035242 IDA
    histone H3R17 methyltransferase activity GO:0035642 IDA
    histone methyltransferase activity GO:0042054 IDA
    histone methyltransferase activity GO:0042054 ISO
    protein homodimerization activity GO:0042803 IPI
    histone H4R3 methyltransferase activity GO:0044020 IEA
    histone H3K37 methyltransferase activity GO:0062122 IEA
    lysine-acetylated histone binding GO:0070577 IDA
    histone H3R2 methyltransferase activity GO:0070611 IBA
    histone H3R2 methyltransferase activity GO:0070611 IDA
    histone H3R2 methyltransferase activity GO:0070611 ISO
    histone H3R8 methyltransferase activity GO:0140592 IEA
    histone H3K56 methyltransferase activity GO:0140759 IEA
    histone H3R26 methyltransferase activity GO:0140903 IDA
    histone H4K12 methyltransferase activity GO:0140984 IEA
    histone H2AQ104 methyltransferase activity GO:1990259 IEA
Biological Process
    regulation of growth plate cartilage chondrocyte proliferation GO:0003420 IMP
    chromatin remodeling GO:0006338 IBA
    chromatin remodeling GO:0006338 IEA
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of DNA-templated transcription GO:0006355 IDA
    protein methylation GO:0006479 IMP
    cell population proliferation GO:0008283 IMP
    positive regulation of cell population proliferation GO:0008284 IMP
    positive regulation of cell population proliferation GO:0008284 ISO
    nuclear receptor-mediated steroid hormone signaling pathway GO:0030518 IDA
    estrogen receptor signaling pathway GO:0030520 IGI
    negative regulation of protein binding GO:0032091 IDA
    methylation GO:0032259 IEA
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 IDA
    positive regulation of fat cell differentiation GO:0045600 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    response to cAMP GO:0051591 ISO
    endochondral bone morphogenesis GO:0060350 IMP
    protein localization to chromatin GO:0071168 IMP
    replication fork reversal GO:0071932 ISO
    replication fork reversal GO:0071932 ISS
    positive regulation of epithelial cell apoptotic process GO:1904037 ISO
    negative regulation of dendrite development GO:2000171 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    protein-containing complex GO:0032991 IDA
    nuclear replication fork GO:0043596 ISO
    nuclear replication fork GO:0043596 ISS
    RNA polymerase II transcription regulator complex GO:0090575 IDA
 Experiment description of studies that identified Carm1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Carm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ncoa3  
Biochemical Activity Mus musculus
Reconstituted Complex Mus musculus
2 RABGAP1 23637
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 Tnik  
Affinity Capture-MS Mus musculus
4 Ncoa1  
Reconstituted Complex Mus musculus
5 Mef2c  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Mycbp2 105689
Affinity Capture-MS Mus musculus
8 Fitm2  
Negative Genetic Mus musculus
9 Herc2  
Affinity Capture-MS Mus musculus
10 Ncoa2  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
11 Ddb1 13194
Affinity Capture-MS Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
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