Gene description for Nampt
Gene name nicotinamide phosphoribosyltransferase
Gene symbol Nampt
Other names/aliases 1110035O14Rik
AI314458
AI480535
NAmPRTase
Pbef
Pbef1
Visfatin
Species Mus musculus
 Database cross references - Nampt
ExoCarta ExoCarta_59027
Vesiclepedia VP_59027
Entrez Gene 59027
UniProt Q99KQ4  
 Nampt identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nampt
Molecular Function
    nucleotide binding GO:0000166 ISO
    cytokine activity GO:0005125 IEA
    cytokine activity GO:0005125 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    nicotinamide phosphoribosyltransferase activity GO:0047280 IBA
    nicotinamide phosphoribosyltransferase activity GO:0047280 IDA
    nicotinamide phosphoribosyltransferase activity GO:0047280 ISO
    heterocyclic compound binding GO:1901363 ISO
Biological Process
    microglial cell activation GO:0001774 ISO
    signal transduction GO:0007165 IEA
    circadian rhythm GO:0007623 IEP
    NAD biosynthetic process GO:0009435 IBA
    NAD biosynthetic process GO:0009435 IDA
    NAD biosynthetic process GO:0009435 IEA
    negative regulation of autophagy GO:0010507 ISO
    regulation of lung blood pressure GO:0014916 ISO
    circadian regulation of gene expression GO:0032922 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of smooth muscle cell proliferation GO:0048661 ISO
    negative regulation of cellular senescence GO:2000773 ISO
Subcellular Localization
    extracellular space GO:0005615 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    cell junction GO:0030054 IEA
    cell junction GO:0030054 ISO
 Experiment description of studies that identified Nampt in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nampt
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
2 Tia1  
Affinity Capture-MS Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here