Gene description for Hacd3
Gene name 3-hydroxyacyl-CoA dehydratase 3
Gene symbol Hacd3
Other names/aliases 4930523M17Rik
AW742319
B-ind1
Hcad3
Hspc121
Ptplad1
Species Mus musculus
 Database cross references - Hacd3
ExoCarta ExoCarta_57874
Vesiclepedia VP_57874
Entrez Gene 57874
UniProt Q8K2C9  
 Hacd3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hacd3
Molecular Function
    protein binding GO:0005515 IPI
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 IBA
    enzyme binding GO:0019899 ISO
    enzyme binding GO:0019899 ISS
    very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity GO:0102158 ISO
    very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity GO:0102158 ISS
Biological Process
    JNK cascade GO:0007254 IDA
    Rho protein signal transduction GO:0007266 IDA
    sphingolipid biosynthetic process GO:0030148 IBA
    fatty acid elongation GO:0030497 IBA
    fatty acid elongation GO:0030497 ISO
    fatty acid elongation GO:0030497 ISS
    very long-chain fatty acid biosynthetic process GO:0042761 IBA
    very long-chain fatty acid biosynthetic process GO:0042761 ISO
    very long-chain fatty acid biosynthetic process GO:0042761 ISS
    positive regulation of viral genome replication GO:0045070 IEA
    positive regulation of viral genome replication GO:0045070 ISO
    positive regulation by virus of viral protein levels in host cell GO:0046726 IEA
    positive regulation by virus of viral protein levels in host cell GO:0046726 ISO
    negative regulation of intracellular signal transduction GO:1902532 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
 Experiment description of studies that identified Hacd3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hacd3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Kcna3  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Cul7  
Co-fractionation Mus musculus
5 Atg16l1  
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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