Gene description for Piwil2
Gene name piwi-like RNA-mediated gene silencing 2
Gene symbol Piwil2
Other names/aliases Piwil1l
mili
Species Mus musculus
 Database cross references - Piwil2
ExoCarta ExoCarta_57746
Vesiclepedia VP_57746
Entrez Gene 57746
UniProt Q8CDG1  
 Piwil2 identified in sEVs derived from the following tissue/cell type
Fibroblasts 23260141    
Mast cells 17486113    
 Gene ontology annotations for Piwil2
Molecular Function
    mRNA binding GO:0003729 IDA
    RNA endonuclease activity GO:0004521 IBA
    RNA endonuclease activity GO:0004521 IDA
    RNA endonuclease activity GO:0004521 IMP
    protein binding GO:0005515 IPI
    piRNA binding GO:0034584 IBA
    piRNA binding GO:0034584 IDA
    piRNA binding GO:0034584 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    pachytene GO:0000239 IGI
    pachytene GO:0000239 IMP
    spermatogenesis GO:0007283 IBA
    spermatogenesis GO:0007283 IMP
    germ-line stem cell population maintenance GO:0030718 IMP
    regulatory ncRNA-mediated gene silencing GO:0031047 IBA
    regulatory ncRNA-mediated gene silencing GO:0031047 IMP
    piRNA processing GO:0034587 IBA
    piRNA processing GO:0034587 IDA
    piRNA processing GO:0034587 IMP
    negative regulation of circadian rhythm GO:0042754 IMP
    negative regulation of circadian rhythm GO:0042754 ISO
    oogenesis GO:0048477 IEP
    rhythmic process GO:0048511 IEA
    meiotic cell cycle GO:0051321 IEA
    positive regulation of meiosis I GO:0060903 IMP
    secondary piRNA processing GO:0140965 IDA
    secondary piRNA processing GO:0140965 IMP
    piRNA-mediated gene silencing by mRNA destabilization GO:0140991 IMP
    transposable element silencing by heterochromatin formation GO:0141005 IMP
    transposable element silencing by piRNA-mediated heterochromatin formation GO:0141006 IMP
    transposable element silencing by mRNA destabilization GO:0141008 IMP
    transposable element silencing by piRNA-mediated DNA methylation GO:0141196 IMP
    positive regulation of cytoplasmic translation GO:2000767 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    perinucleolar chromocenter GO:0010370 IDA
    chromatoid body GO:0033391 IDA
    P granule GO:0043186 IBA
    P granule GO:0043186 IDA
    pi-body GO:0071546 IDA
    dense body GO:0097433 IDA
    PET complex GO:1990923 IDA
 Experiment description of studies that identified Piwil2 in sEVs
1
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Piwil2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mael  
Affinity Capture-Western Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
3 Piwil4  
Co-fractionation Mus musculus
4 Tdrd1  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Piwil2 is involved
No pathways found





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