Experiment description of studies that identified Eif5 in sEVs

1 Experiment ID 95 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

2 Experiment ID 96 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

3 Experiment ID 97 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

4 Experiment ID 98 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

Protein-protein interactions for Eif5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 EIF2S2 8894 Reconstituted Complex Homo sapiens Far Western Homo sapiens View the network

Pathways in which Eif5 is involved

No pathways found