Gene description for Hist1h1b
Gene name histone cluster 1, H1b
Gene symbol Hist1h1b
Other names/aliases H1.5
H1B
H1f5
H1s-3
Species Mus musculus
 Database cross references - Hist1h1b
ExoCarta ExoCarta_56702
Vesiclepedia VP_56702
Entrez Gene 56702
UniProt P43276  
 Hist1h1b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hist1h1b
Molecular Function
    DNA binding GO:0003677 IDA
    double-stranded DNA binding GO:0003690 IBA
    protein binding GO:0005515 IPI
    structural constituent of chromatin GO:0030527 IEA
    chromatin DNA binding GO:0031490 IEA
    chromatin DNA binding GO:0031490 ISO
    nucleosomal DNA binding GO:0031492 IBA
    histone deacetylase binding GO:0042826 IEA
    histone deacetylase binding GO:0042826 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    chromatin organization GO:0006325 ISO
    nucleosome assembly GO:0006334 IEA
    muscle organ development GO:0007517 IPI
    chromosome condensation GO:0030261 IBA
    negative regulation of DNA recombination GO:0045910 IBA
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    establishment of protein localization to chromatin GO:0071169 IEA
    establishment of protein localization to chromatin GO:0071169 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    nucleosome GO:0000786 IEA
    euchromatin GO:0000791 IBA
    heterochromatin GO:0000792 IEA
    heterochromatin GO:0000792 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    chromosome GO:0005694 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Hist1h1b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hist1h1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Msx1  
Affinity Capture-Western Mus musculus
2 Cdk2  
Biochemical Activity Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Cdk4  
Biochemical Activity Mus musculus
5 Tfe3  
Affinity Capture-MS Mus musculus
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 Ddb1 13194
Affinity Capture-MS Mus musculus
8 JADE1  
Affinity Capture-MS Homo sapiens
9 Fancd2  
Affinity Capture-MS Mus musculus
10 Mettl21e  
Affinity Capture-MS Mus musculus
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