Experiment description of studies that identified TMPRSS4 in sEVs

1 Experiment ID 1203 MISEV standards ✔ EM Biophysical techniques ✔ SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

2 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for TMPRSS4

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SUSD3 203328 Two-hybrid Homo sapiens 2 CLRN1   Two-hybrid Homo sapiens 3 FAM209A 200232 Two-hybrid Homo sapiens 4 GPX8 493869 Two-hybrid Homo sapiens 5 SYNE4   Two-hybrid Homo sapiens 6 FNDC9   Two-hybrid Homo sapiens 7 SIGLEC12   Two-hybrid Homo sapiens 8 BNIP3L   Two-hybrid Homo sapiens 9 TMEM79   Two-hybrid Homo sapiens 10 CLEC7A   Two-hybrid Homo sapiens 11 CCDC155   Two-hybrid Homo sapiens 12 RASGRP4   Two-hybrid Homo sapiens 13 ERVFRD-1   Two-hybrid Homo sapiens View the network

Pathways in which TMPRSS4 is involved

No pathways found