Gene description for Slc15a1
Gene name solute carrier family 15 (oligopeptide transporter), member 1
Gene symbol Slc15a1
Other names/aliases D630032F02
PECT1
PEPT1
Species Mus musculus
 Database cross references - Slc15a1
ExoCarta ExoCarta_56643
Vesiclepedia VP_56643
Entrez Gene 56643
UniProt Q9JIP7  
 Slc15a1 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Slc15a1
Molecular Function
    proton-dependent oligopeptide secondary active transmembrane transporter activity GO:0005427 IDA
    peptide:proton symporter activity GO:0015333 IDA
    peptide:proton symporter activity GO:0015333 ISO
    channel inhibitor activity GO:0016248 ISO
    tripeptide transmembrane transporter activity GO:0042937 IEA
    tripeptide transmembrane transporter activity GO:0042937 ISO
    dipeptide transmembrane transporter activity GO:0071916 IBA
    dipeptide transmembrane transporter activity GO:0071916 IDA
    dipeptide transmembrane transporter activity GO:0071916 ISO
Biological Process
    oligopeptide transport GO:0006857 IDA
    protein transport GO:0015031 IEA
    tripeptide transport GO:0042939 IEA
    negative regulation of amino acid transport GO:0051956 ISO
    dipeptide import across plasma membrane GO:0140206 IBA
    dipeptide import across plasma membrane GO:0140206 IDA
    dipeptide import across plasma membrane GO:0140206 ISO
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    brush border GO:0005903 IDA
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
 Experiment description of studies that identified Slc15a1 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Slc15a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Usp7  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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