Gene description for Rhog
Gene name ras homolog gene family, member G
Gene symbol Rhog
Other names/aliases 2810426G09Rik
Arhg
Sid10750
Species Mus musculus
 Database cross references - Rhog
ExoCarta ExoCarta_56212
Vesiclepedia VP_56212
Entrez Gene 56212
UniProt P84096  
 Rhog identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Microglia 16081791    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rhog
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    protein kinase binding GO:0019901 IBA
Biological Process
    actin filament organization GO:0007015 IBA
    establishment or maintenance of cell polarity GO:0007163 IBA
    Rho protein signal transduction GO:0007266 ISO
    regulation of cell shape GO:0008360 IBA
    Rac protein signal transduction GO:0016601 IBA
    Rac protein signal transduction GO:0016601 ISO
    actin cytoskeleton organization GO:0030036 ISO
    cortical cytoskeleton organization GO:0030865 IBA
    regulation of actin cytoskeleton organization GO:0032956 IBA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    cell chemotaxis GO:0060326 IBA
    cell chemotaxis GO:0060326 ISO
    cell chemotaxis GO:0060326 ISS
    activation of GTPase activity GO:0090630 ISO
    activation of GTPase activity GO:0090630 ISS
    regulation of postsynapse assembly GO:0150052 IEA
    regulation of postsynapse assembly GO:0150052 ISO
    regulation of ruffle assembly GO:1900027 IDA
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 ISS
Subcellular Localization
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    cytoplasmic vesicle GO:0031410 IBA
    cell projection GO:0042995 IBA
    postsynapse GO:0098794 IEA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rhog in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rhog
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mapt  
Affinity Capture-MS Mus musculus
2 SMAD4  
Affinity Capture-Luminescence Homo sapiens
3 Fancd2  
Affinity Capture-MS Mus musculus
4 Atg16l1  
Affinity Capture-MS Mus musculus
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