Gene description for Slc1a4
Gene name solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
Gene symbol Slc1a4
Other names/aliases ASCT-1
ASCT1
AW045657
SATT
Species Mus musculus
 Database cross references - Slc1a4
ExoCarta ExoCarta_55963
Vesiclepedia VP_55963
Entrez Gene 55963
UniProt O35874  
 Slc1a4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc1a4
Molecular Function
    chloride channel activity GO:0005254 IEA
    chloride channel activity GO:0005254 ISO
    neutral L-amino acid transmembrane transporter activity GO:0015175 IDA
    L-alanine transmembrane transporter activity GO:0015180 IBA
    L-alanine transmembrane transporter activity GO:0015180 IEA
    L-alanine transmembrane transporter activity GO:0015180 ISO
    L-aspartate transmembrane transporter activity GO:0015183 IBA
    L-aspartate transmembrane transporter activity GO:0015183 IDA
    L-cystine transmembrane transporter activity GO:0015184 IEA
    L-cystine transmembrane transporter activity GO:0015184 ISO
    L-proline transmembrane transporter activity GO:0015193 IEA
    L-proline transmembrane transporter activity GO:0015193 ISO
    L-serine transmembrane transporter activity GO:0015194 IBA
    L-serine transmembrane transporter activity GO:0015194 IDA
    L-serine transmembrane transporter activity GO:0015194 ISO
    L-threonine transmembrane transporter activity GO:0015195 IBA
    L-threonine transmembrane transporter activity GO:0015195 IEA
    L-threonine transmembrane transporter activity GO:0015195 ISO
    symporter activity GO:0015293 IEA
    L-hydroxyproline transmembrane transporter activity GO:0034590 IEA
    L-hydroxyproline transmembrane transporter activity GO:0034590 ISO
Biological Process
    acidic amino acid transport GO:0015800 IEA
    L-alanine transport GO:0015808 ISO
    L-cystine transport GO:0015811 IEA
    L-cystine transport GO:0015811 ISO
    L-glutamate transmembrane transport GO:0015813 IBA
    proline transport GO:0015824 IEA
    proline transport GO:0015824 ISO
    L-serine transport GO:0015825 IBA
    L-serine transport GO:0015825 ISO
    threonine transport GO:0015826 IEA
    threonine transport GO:0015826 ISO
    hydroxyproline transport GO:0034589 IEA
    hydroxyproline transport GO:0034589 ISO
    proline transmembrane transport GO:0035524 IEA
    cognition GO:0050890 IEA
    cognition GO:0050890 ISO
    serine import across plasma membrane GO:0098718 IDA
    L-aspartate import across plasma membrane GO:0140009 IDA
    chloride transmembrane transport GO:1902476 IEA
    L-serine import across plasma membrane GO:1903812 IEA
    L-serine import across plasma membrane GO:1903812 ISO
    L-alanine import across plasma membrane GO:1904273 IEA
    L-alanine import across plasma membrane GO:1904273 ISO
Subcellular Localization
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    intermediate filament GO:0005882 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    melanosome GO:0042470 IEA
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Slc1a4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc1a4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here