Gene description for Ezr
Gene name ezrin
Gene symbol Ezr
Other names/aliases Vil2
Species Rattus norvegicus
 Database cross references - Ezr
ExoCarta ExoCarta_54319
Entrez Gene 54319
UniProt P31977  
 Ezr identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Ezr
Molecular Function
    structural molecule activity GO:0005198 TAS
    actin filament binding GO:0051015 ISS
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    protein complex binding GO:0032403 IPI
    cell adhesion molecule binding GO:0050839 ISS
    poly(A) RNA binding GO:0044822 ISO
Biological Process
    positive regulation of cellular protein catabolic process GO:1903364 ISO
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 ISO
    positive regulation of gene expression GO:0010628 ISO
    filopodium assembly GO:0046847 ISO
    sphingosine-1-phosphate signaling pathway GO:0003376 ISO
    phosphatidylinositol-mediated signaling GO:0048015 ISO
    positive regulation of multicellular organism growth GO:0040018 ISO
    positive regulation of protein secretion GO:0050714 ISO
    regulation of cell shape GO:0008360 IEA
    intestinal D-glucose absorption GO:0001951 ISO
    regulation of organelle assembly GO:1902115 ISO
    actin cytoskeleton reorganization GO:0031532 ISO
    epithelial cell differentiation GO:0030855 IEP
    leukocyte cell-cell adhesion GO:0007159 ISO
    membrane to membrane docking GO:0022614 ISO
    positive regulation of protein localization to early endosome GO:1902966 ISO
    microvillus assembly GO:0030033 ISO
    receptor internalization GO:0031623 ISO
    actin filament bundle assembly GO:0051017 ISS
    terminal web assembly GO:1902896 ISO
    establishment of epithelial cell apical/basal polarity GO:0045198 ISO
    regulation of microvillus length GO:0032532 ISO
    establishment or maintenance of apical/basal cell polarity GO:0035088 ISO
    positive regulation of early endosome to late endosome transport GO:2000643 ISO
    establishment of endothelial barrier GO:0061028 ISO
Subcellular Localization
    filopodium GO:0030175 ISO
    actin cytoskeleton GO:0015629 ISS
    extracellular exosome GO:0070062 ISO
    focal adhesion GO:0005925 ISO
    actin filament GO:0005884 ISS
    extrinsic component of membrane GO:0019898 ISS
    cell periphery GO:0071944 ISO
    microspike GO:0044393 IDA
    astrocyte projection GO:0097449 IDA
    myelin sheath GO:0043209 ISO
    extracellular space GO:0005615 ISO
    brush border GO:0005903 ISO
    cytoplasmic side of apical plasma membrane GO:0098592 ISO
    uropod GO:0001931 ISO
    membrane raft GO:0045121 IDA
    endosome GO:0005768 ISO
    apical plasma membrane GO:0016324 ISO
    microvillus GO:0005902 ISO
    ruffle GO:0001726 ISO
    ciliary basal body GO:0036064 ISO
    membrane GO:0016020 ISO
    apical part of cell GO:0045177 ISO
    T-tubule GO:0030315 IDA
    vesicle GO:0031982 ISO
    microvillus membrane GO:0031528 IDA
    cell tip GO:0051286 IDA
    ruffle membrane GO:0032587 IEA
    cell cortex GO:0005938 IEA
    basolateral plasma membrane GO:0016323 IDA
    intracellular GO:0005622 ISO
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISO
    cell body GO:0044297 IDA
    Schwann cell microvillus GO:0097454 IDA
 Experiment description of studies that identified Ezr in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ezr
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ezr is involved
PathwayEvidenceSource
Netrin-1 signaling IEA Reactome
Recycling pathway of L1 IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here