Gene description for Car3
Gene name carbonic anhydrase 3
Gene symbol Car3
Other names/aliases Ca3
Species Rattus norvegicus
 Database cross references - Car3
ExoCarta ExoCarta_54232
Entrez Gene 54232
UniProt P14141  
 Car3 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Car3
Molecular Function
    carbonate dehydratase activity GO:0004089 TAS
    zinc ion binding GO:0008270 IEA
    phosphatase activity GO:0016791 IDA
    nickel cation binding GO:0016151 ISO
Biological Process
    response to oxidative stress GO:0006979 IMP
    response to ethanol GO:0045471 IEP
    dephosphorylation GO:0016311 IDA
    one-carbon metabolic process GO:0006730 IEA
Subcellular Localization
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Car3 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Car3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Car3 is involved
PathwayEvidenceSource
Reversible hydration of carbon dioxide IEA Reactome





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