Gene description for ACTN4
Gene name actinin, alpha 4
Gene symbol ACTN4
Other names/aliases -
Species Bos taurus
 Database cross references - ACTN4
ExoCarta ExoCarta_522269
Entrez Gene 522269
UniProt A5D7D1  
 ACTN4 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for ACTN4
Molecular Function
    poly(A) RNA binding GO:0044822 IEA
    retinoic acid receptor binding GO:0042974 IEA
    calcium ion binding GO:0005509 IEA
    actin filament binding GO:0051015 IEA
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 ISS
    nucleoside binding GO:0001882 IEA
Biological Process
    positive regulation of cellular component movement GO:0051272 IEA
    peroxisome proliferator activated receptor signaling pathway GO:0035357 ISS
    negative regulation of cellular component movement GO:0051271 IEA
    bicellular tight junction assembly GO:0070830 IEA
    retinoic acid receptor signaling pathway GO:0048384 ISS
    protein transport GO:0015031 IEA
    protein localization to bicellular tight junction GO:1902396 IEA
    regulation of nucleic acid-templated transcription GO:1903506 IEA
    actin filament bundle assembly GO:0051017 IEA
    positive regulation of pinocytosis GO:0048549 IEA
Subcellular Localization
    cell-cell junction GO:0005911 IEA
    protein complex GO:0043234 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    cytoplasm GO:0005737 ISS
    extracellular space GO:0005615 IEA
    pseudopodium GO:0031143 IEA
    nucleus GO:0005634 ISS
    stress fiber GO:0001725 IEA
    extracellular exosome GO:0070062 IEA
    ribonucleoprotein complex GO:0030529 ISS
    focal adhesion GO:0005925 IEA
    Z disc GO:0030018 IEA
    cortical cytoskeleton GO:0030863 IEA
 Experiment description of studies that identified ACTN4 in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACTN4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ACTN4 is involved
PathwayEvidenceSource
Nephrin interactions IEA Reactome
Platelet degranulation IEA Reactome





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