Gene description for Hist1h1c
Gene name histone cluster 1, H1c
Gene symbol Hist1h1c
Other names/aliases 0610008C09Rik
H1.2
H1c
H1f2
H1var1
His1a
Species Mus musculus
 Database cross references - Hist1h1c
ExoCarta ExoCarta_50708
Vesiclepedia VP_50708
Entrez Gene 50708
UniProt P15864  
 Hist1h1c identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hist1h1c
Molecular Function
    DNA binding GO:0003677 IDA
    double-stranded DNA binding GO:0003690 IBA
    protein binding GO:0005515 IPI
    structural constituent of chromatin GO:0030527 IMP
    chromatin DNA binding GO:0031490 IEA
    chromatin DNA binding GO:0031490 ISO
    nucleosomal DNA binding GO:0031492 IBA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    chromatin organization GO:0006325 IMP
    nucleosome assembly GO:0006334 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    chromosome condensation GO:0030261 IBA
    negative regulation of DNA recombination GO:0045910 IBA
Subcellular Localization
    nucleosome GO:0000786 IEA
    euchromatin GO:0000791 IBA
    euchromatin GO:0000791 IDA
    euchromatin GO:0000791 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Hist1h1c in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hist1h1c
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cdk4  
Biochemical Activity Mus musculus
2 Cdk2  
Biochemical Activity Mus musculus
3 Elf5  
Affinity Capture-MS Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Tfe3  
Affinity Capture-MS Mus musculus
7 Pou5f1  
Affinity Capture-MS Mus musculus
8 Ddb1 13194
Affinity Capture-MS Mus musculus
9 Nanog  
Affinity Capture-MS Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
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