Gene description for Aco1
Gene name aconitase 1, soluble
Gene symbol Aco1
Other names/aliases AH
Acon1
IRP1
Species Rattus norvegicus
 Database cross references - Aco1
ExoCarta ExoCarta_50655
Vesiclepedia VP_50655
Entrez Gene 50655
UniProt Q63270  
 Aco1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Aco1
Molecular Function
    mRNA regulatory element binding translation repressor activity GO:0000900 ISO
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 ISO
    aconitate hydratase activity GO:0003994 IBA
    aconitate hydratase activity GO:0003994 IDA
    aconitate hydratase activity GO:0003994 IEA
    aconitate hydratase activity GO:0003994 ISO
    aconitate hydratase activity GO:0003994 ISS
    iron-responsive element binding GO:0030350 IBA
    iron-responsive element binding GO:0030350 IDA
    iron-responsive element binding GO:0030350 IEA
    iron-responsive element binding GO:0030350 ISO
    iron-responsive element binding GO:0030350 ISS
    metal ion binding GO:0046872 IEA
    mRNA 5'-UTR binding GO:0048027 IDA
    iron-sulfur cluster binding GO:0051536 IDA
    iron-sulfur cluster binding GO:0051536 ISO
    3 iron, 4 sulfur cluster binding GO:0051538 IBA
    3 iron, 4 sulfur cluster binding GO:0051538 IEA
    3 iron, 4 sulfur cluster binding GO:0051538 ISO
    4 iron, 4 sulfur cluster binding GO:0051539 IBA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 ISO
    4 iron, 4 sulfur cluster binding GO:0051539 ISS
Biological Process
    liver development GO:0001889 IEP
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 IEA
    citrate metabolic process GO:0006101 IBA
    citrate metabolic process GO:0006101 IEA
    citrate metabolic process GO:0006101 ISO
    citrate metabolic process GO:0006101 ISS
    regulation of translation GO:0006417 IEA
    regulation of translation GO:0006417 ISO
    intracellular iron ion homeostasis GO:0006879 IDA
    intracellular iron ion homeostasis GO:0006879 IEA
    intracellular iron ion homeostasis GO:0006879 ISO
    post-embryonic development GO:0009791 IEA
    post-embryonic development GO:0009791 ISO
    response to iron(II) ion GO:0010040 IEA
    response to iron(II) ion GO:0010040 ISO
    response to iron(II) ion GO:0010040 ISS
    regulation of gene expression GO:0010468 ISO
    negative regulation of translation GO:0017148 IEA
    intestinal absorption GO:0050892 IEA
    intestinal absorption GO:0050892 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Aco1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Aco1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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