Gene description for Pdcd6ip
Gene name programmed cell death 6 interacting protein
Gene symbol Pdcd6ip
Other names/aliases AIP1
Alix
RGD1561176
Species Rattus norvegicus
 Database cross references - Pdcd6ip
ExoCarta ExoCarta_501083
Vesiclepedia VP_501083
Entrez Gene 501083
UniProt Q9QZA2  
 Pdcd6ip identified in sEVs derived from the following tissue/cell type
Cortical neurones 16446100    
Hepatocytes 19367702    
Hippocampal neuronal cells 34654817    
Hippocampal neuronal cells 34654817    
Neural stem cells 31848325    
Neural stem cells 31848325    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Schwann cells 36717543    
Urine 20535238    
 Gene ontology annotations for Pdcd6ip
Molecular Function
    SH3 domain binding GO:0017124 IDA
    proteinase activated receptor binding GO:0031871 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    protein dimerization activity GO:0046983 IEA
    protein dimerization activity GO:0046983 ISO
    calcium-dependent protein binding GO:0048306 ISO
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    mitotic cytokinesis GO:0000281 ISO
    mitotic cytokinesis GO:0000281 ISS
    actomyosin contractile ring assembly GO:0000915 IEA
    actomyosin contractile ring assembly GO:0000915 ISO
    actomyosin contractile ring assembly GO:0000915 ISS
    apoptotic process GO:0006915 IEA
    nucleus organization GO:0006997 ISO
    mitotic metaphase chromosome alignment GO:0007080 ISO
    regulation of centrosome duplication GO:0010824 ISO
    protein transport GO:0015031 IEA
    macroautophagy GO:0016236 IEA
    macroautophagy GO:0016236 ISO
    macroautophagy GO:0016236 ISS
    viral budding via host ESCRT complex GO:0039702 ISO
    maintenance of epithelial cell apical/basal polarity GO:0045199 IEA
    maintenance of epithelial cell apical/basal polarity GO:0045199 ISO
    maintenance of epithelial cell apical/basal polarity GO:0045199 ISS
    viral budding GO:0046755 ISO
    protein homooligomerization GO:0051260 ISO
    midbody abscission GO:0061952 ISO
    bicellular tight junction assembly GO:0070830 IEA
    bicellular tight junction assembly GO:0070830 ISO
    bicellular tight junction assembly GO:0070830 ISS
    regulation of membrane permeability GO:0090559 IEA
    regulation of membrane permeability GO:0090559 ISO
    regulation of membrane permeability GO:0090559 ISS
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 ISO
    extracellular exosome biogenesis GO:0097734 IEA
    extracellular exosome biogenesis GO:0097734 ISO
    extracellular exosome biogenesis GO:0097734 ISS
    regulation of mitotic spindle assembly GO:1901673 ISO
    positive regulation of exosomal secretion GO:1903543 ISO
    regulation of extracellular exosome assembly GO:1903551 ISO
    positive regulation of extracellular exosome assembly GO:1903553 ISO
Subcellular Localization
    immunological synapse GO:0001772 ISO
    endosome GO:0005768 IBA
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    bicellular tight junction GO:0005923 IEA
    melanosome GO:0042470 IEA
    actomyosin GO:0042641 IEA
    actomyosin GO:0042641 ISO
    actomyosin GO:0042641 ISS
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    extracellular exosome GO:0070062 ISS
    endoplasmic reticulum exit site GO:0070971 ISO
    Flemming body GO:0090543 ISO
    Flemming body GO:0090543 ISS
 Experiment description of studies that identified Pdcd6ip in sEVs
1
Experiment ID 30
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 1160
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34654817    
Organism Rattus norvegicus
Experiment description Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors
Authors "Xia W, Xie J, Cai Z, Liu X, Wen J, Cui ZK, Zhao R, Zhou X, Chen J, Mao X, Gu Z, Zou Z, Zou Z, Zhang Y, Zhao M, Mac M, Song Q, Bai X."
Journal name Nat Commun
Publication year 2021
Sample Hippocampal neuronal cells
Sample name Primary hippocampal neurons - Sprague-Dawley rat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 1161
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34654817    
Organism Rattus norvegicus
Experiment description Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors
Authors "Xia W, Xie J, Cai Z, Liu X, Wen J, Cui ZK, Zhao R, Zhou X, Chen J, Mao X, Gu Z, Zou Z, Zou Z, Zhang Y, Zhao M, Mac M, Song Q, Bai X."
Journal name Nat Commun
Publication year 2021
Sample Hippocampal neuronal cells
Sample name Primary hippocampal neurons - Sprague-Dawley rat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 1217
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 31848325    
Organism Rattus norvegicus
Experiment description Insulin-like growth factor-1 enchances neuroprotective effects of neural stem cell exosomes after spinal cord injury via an miR-219a-2-3p/YY1 mechanism
Authors "Ma K, Xu H, Zhang J, Zhao F, Liang H, Sun H, Li P, Zhang S, Wang R, Chen X."
Journal name Aging
Publication year 2019
Sample Neural stem cells
Sample name Cerebral cortex-derived NSCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RNA sequencing
qRT-PCR
6
Experiment ID 1218
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 31848325    
Organism Rattus norvegicus
Experiment description Insulin-like growth factor-1 enchances neuroprotective effects of neural stem cell exosomes after spinal cord injury via an miR-219a-2-3p/YY1 mechanism
Authors "Ma K, Xu H, Zhang J, Zhao F, Liang H, Sun H, Li P, Zhang S, Wang R, Chen X."
Journal name Aging
Publication year 2019
Sample Neural stem cells
Sample name Cerebral cortex-derived NSCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RNA sequencing
qRT-PCR
7
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
11
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
12
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
13
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
14
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
15
Experiment ID 678
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36717543    
Organism Rattus norvegicus
Experiment description Schwann cell-derived exosomes containing MFG-E8 modify macrophage/microglial polarization for attenuating inflammation via the SOCS3/STAT3 pathway after spinal cord injury
Authors "Ren J, Zhu B, Gu G, Zhang W, Li J, Wang H, Wang M, Song X, Wei Z, Feng S."
Journal name Cell Death Dis
Publication year 2023
Sample Schwann cells
Sample name Sciatic nerve-derived schwann cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Pdcd6ip
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here