Gene description for Dnmt3b
Gene name DNA (cytosine-5-)-methyltransferase 3 beta
Gene symbol Dnmt3b
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dnmt3b
ExoCarta ExoCarta_444985
Entrez Gene 444985
 Dnmt3b identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Dnmt3b
Molecular Function
    DNA binding GO:0003677 ISO
    DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates GO:0051718 IDA
    chromatin binding GO:0003682 IDA
    transcription corepressor activity GO:0003714 ISO
    DNA (cytosine-5-)-methyltransferase activity GO:0003886 ISO
    unmethylated CpG binding GO:0045322 IDA
    methyltransferase activity GO:0008168 ISO
    protein binding GO:0005515 IPI
    histone deacetylase binding GO:0042826 IPI
Biological Process
    protein complex localization GO:0031503 ISO
    response to cocaine GO:0042220 IEP
    cellular response to hyperoxia GO:0071455 IEP
    response to hypoxia GO:0001666 IEP
    S-adenosylmethioninamine metabolic process GO:0046499 IDA
    negative regulation of gene expression, epigenetic GO:0045814 ISO
    response to estradiol GO:0032355 IEP
    cellular response to amino acid stimulus GO:0071230 ISO
    C-5 methylation of cytosine GO:0090116 ISO
    response to toxic substance GO:0009636 IEP
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    response to ionizing radiation GO:0010212 IEP
    cellular response to dexamethasone stimulus GO:0071549 IEP
    response to activity GO:0014823 IEP
    DNA methylation on cytosine within a CG sequence GO:0010424 IDA
    positive regulation of neuron differentiation GO:0045666 IMP
    response to drug GO:0042493 IEP
    negative regulation of histone H3-K9 methylation GO:0051573 ISO
    positive regulation of histone H3-K4 methylation GO:0051571 ISO
    response to vitamin A GO:0033189 IEP
    inactivation of X chromosome by DNA methylation GO:0060821 ISO
    S-adenosylhomocysteine metabolic process GO:0046498 IDA
    DNA methylation GO:0006306 ISO
    methylation-dependent chromatin silencing GO:0006346 ISO
    response to caffeine GO:0031000 IEP
    regulation of gene expression by genetic imprinting GO:0006349 ISO
Subcellular Localization
    cytoplasm GO:0005737 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    heterochromatin GO:0000792 ISO
    chromosome, centromeric region GO:0000775 ISO
    nucleus GO:0005634 ISO
    nuclear heterochromatin GO:0005720 ISO
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Dnmt3b in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Dnmt3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dnmt3b is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here