Gene description for MGAT1
Gene name mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Gene symbol MGAT1
Other names/aliases GLCNAC-TI
GLCT1
GLYT1
GNT-1
GNT-I
MGAT
Species Homo sapiens
 Database cross references - MGAT1
ExoCarta ExoCarta_4245
Entrez Gene 4245
HGNC 7044
MIM 160995
UniProt P26572  
 MGAT1 identified in exosomes derived from the following tissue/cell type
Saliva 19199708    
 Gene ontology annotations for MGAT1
Molecular Function
    acetylglucosaminyltransferase activity GO:0008375 TAS
    metal ion binding GO:0046872 IEA
    alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity GO:0003827 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 TAS
    post-translational protein modification GO:0043687 TAS
    protein glycosylation GO:0006486 TAS
    cellular protein metabolic process GO:0044267 TAS
    in utero embryonic development GO:0001701 IEA
    UDP-N-acetylglucosamine catabolic process GO:0006049 IEA
    protein N-linked glycosylation via asparagine GO:0018279 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 IDA
    extracellular exosome GO:0070062 IDA
    extracellular vesicle GO:1903561 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified MGAT1 in exosomes
1
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for MGAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMAD9  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MGAT1 is involved
PathwayEvidenceSource
N-glycan trimming and elongation in the cis-Golgi TAS Reactome





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