Gene description for H2afy2
Gene name H2A histone family, member Y2
Gene symbol H2afy2
Other names/aliases macroH2A2
Species Mus musculus
 Database cross references - H2afy2
ExoCarta ExoCarta_404634
Vesiclepedia VP_404634
Entrez Gene 404634
UniProt Q8CCK0  
 H2afy2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for H2afy2
Molecular Function
    transcription cis-regulatory region binding GO:0000976 ISO
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IEA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    structural constituent of chromatin GO:0030527 IBA
    structural constituent of chromatin GO:0030527 IEA
    chromatin DNA binding GO:0031490 IEA
    chromatin DNA binding GO:0031490 ISO
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    nucleosome assembly GO:0006334 IEA
    brain development GO:0007420 IEA
    brain development GO:0007420 ISO
    sex-chromosome dosage compensation GO:0007549 ISO
    heterochromatin formation GO:0031507 IBA
    positive regulation of keratinocyte differentiation GO:0045618 IEA
    positive regulation of keratinocyte differentiation GO:0045618 ISO
    negative regulation of gene expression, epigenetic GO:0045814 IEA
    negative regulation of gene expression, epigenetic GO:0045814 ISO
    establishment of protein localization to chromatin GO:0071169 IEA
    establishment of protein localization to chromatin GO:0071169 ISO
    negative regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901837 IBA
    negative regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901837 IEA
    negative regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901837 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    nucleosome GO:0000786 IBA
    nucleosome GO:0000786 IEA
    Barr body GO:0001740 ISO
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified H2afy2 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for H2afy2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ddb1 13194
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which H2afy2 is involved
No pathways found





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