Gene description for Nsfl1c
Gene name NSFL1 (p97) cofactor (p47)
Gene symbol Nsfl1c
Other names/aliases Munc-18c
Stxbp3a
p47
Species Mus musculus
 Database cross references - Nsfl1c
ExoCarta ExoCarta_386649
Vesiclepedia VP_386649
Entrez Gene 386649
UniProt Q9CZ44  
 Nsfl1c identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nsfl1c
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 ISO
    lipid binding GO:0008289 IEA
    ubiquitin binding GO:0043130 IBA
    ubiquitin binding GO:0043130 ISO
    ATPase binding GO:0051117 ISO
Biological Process
    autophagosome assembly GO:0000045 IBA
    establishment of mitotic spindle orientation GO:0000132 IEA
    establishment of mitotic spindle orientation GO:0000132 ISO
    Golgi organization GO:0007030 IBA
    Golgi organization GO:0007030 ISO
    nuclear membrane reassembly GO:0031468 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    positive regulation of mitotic centrosome separation GO:0046604 IEA
    positive regulation of mitotic centrosome separation GO:0046604 ISO
    membrane fusion GO:0061025 IBA
    membrane fusion GO:0061025 ISO
    negative regulation of protein localization to centrosome GO:1904780 IEA
    negative regulation of protein localization to centrosome GO:1904780 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    chromosome GO:0005694 IEA
    cytoplasm GO:0005737 NAS
    Golgi stack GO:0005795 IEA
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    spindle pole centrosome GO:0031616 ISO
    VCP-NSFL1C complex GO:1990730 IPI
    VCP-NSFL1C complex GO:1990730 ISO
 Experiment description of studies that identified Nsfl1c in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nsfl1c
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Ubqln2  
Co-fractionation Mus musculus
4 Ubxn6  
Co-fractionation Mus musculus
5 Ubqln4  
Co-fractionation Mus musculus
6 Ubfd1  
Co-fractionation Mus musculus
7 Vcpip1  
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here