Gene description for Rac2
Gene name ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
Gene symbol Rac2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rac2
ExoCarta ExoCarta_366957
Vesiclepedia VP_366957
Entrez Gene 366957
 Rac2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rac2
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 ISO
    protein kinase regulator activity GO:0019887 ISO
    protein kinase binding GO:0019901 IBA
Biological Process
    chemotaxis GO:0006935 IBA
    chemotaxis GO:0006935 ISO
    actin filament organization GO:0007015 IBA
    actin filament organization GO:0007015 ISO
    establishment or maintenance of cell polarity GO:0007163 IBA
    signal transduction GO:0007165 IBA
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    small GTPase-mediated signal transduction GO:0007264 IEA
    cell population proliferation GO:0008283 ISO
    regulation of cell shape GO:0008360 IBA
    positive regulation of lamellipodium assembly GO:0010592 IBA
    positive regulation of lamellipodium assembly GO:0010592 ISO
    regulation of cell-substrate adhesion GO:0010810 ISO
    cell projection assembly GO:0030031 ISO
    actin cytoskeleton organization GO:0030036 IDA
    actin cytoskeleton organization GO:0030036 ISO
    cortical cytoskeleton organization GO:0030865 IBA
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of T cell proliferation GO:0042129 ISO
    erythrocyte enucleation GO:0043131 ISO
    regulation of mast cell degranulation GO:0043304 ISO
    bone resorption GO:0045453 IDA
    regulation of respiratory burst GO:0060263 ISO
    regulation of mast cell chemotaxis GO:0060753 ISO
    mast cell proliferation GO:0070662 ISO
    positive regulation of mast cell proliferation GO:0070668 ISO
    lymphocyte aggregation GO:0071593 ISO
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
    regulation of neutrophil migration GO:1902622 IBA
    regulation of neutrophil migration GO:1902622 ISO
    positive regulation of protein targeting to mitochondrion GO:1903955 ISO
Subcellular Localization
    nuclear envelope GO:0005635 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 IBA
    actin filament GO:0005884 ISO
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 ISO
    lamellipodium GO:0030027 ISO
    cytoplasmic vesicle GO:0031410 IBA
    cell projection GO:0042995 IBA
 Experiment description of studies that identified Rac2 in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rac2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rac2 is involved
No pathways found





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