Gene description for Tmem165
Gene name transmembrane protein 165
Gene symbol Tmem165
Other names/aliases Tparl
Species Rattus norvegicus
 Database cross references - Tmem165
ExoCarta ExoCarta_364137
Vesiclepedia VP_364137
Entrez Gene 364137
UniProt Q4V899  
 Tmem165 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Tmem165
Molecular Function
    manganese ion transmembrane transporter activity GO:0005384 IBA
    calcium ion transmembrane transporter activity GO:0015085 IBA
    antiporter activity GO:0015297 IEA
Biological Process
    protein N-linked glycosylation GO:0006487 ISO
    protein N-linked glycosylation GO:0006487 ISS
    calcium ion transport GO:0006816 ISO
    calcium ion transport GO:0006816 ISS
    manganese ion transport GO:0006828 ISO
    manganese ion transport GO:0006828 ISS
    intracellular calcium ion homeostasis GO:0006874 ISO
    intracellular calcium ion homeostasis GO:0006874 ISS
    Golgi calcium ion homeostasis GO:0032468 IBA
    Golgi calcium ion transport GO:0032472 IBA
    Golgi calcium ion transport GO:0032472 ISO
    Golgi calcium ion transport GO:0032472 ISS
    regulation of lysosomal lumen pH GO:0035751 ISO
    regulation of lysosomal lumen pH GO:0035751 ISS
    calcium ion transmembrane transport GO:0070588 IBA
    manganese ion transmembrane transport GO:0071421 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    trans-Golgi network membrane GO:0032588 ISO
    trans-Golgi network membrane GO:0032588 ISS
    cis-Golgi network membrane GO:0033106 ISO
    cis-Golgi network membrane GO:0033106 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Tmem165 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tmem165
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tmem165 is involved
No pathways found





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