Gene description for Agfg1
Gene name ArfGAP with FG repeats 1
Gene symbol Agfg1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Agfg1
ExoCarta ExoCarta_363266
Vesiclepedia VP_363266
Entrez Gene 363266
UniProt Q4KLH5  
 Agfg1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Agfg1
Molecular Function
    DNA binding GO:0003677 IEA
    GTPase activator activity GO:0005096 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    acrosome assembly GO:0001675 IBA
    acrosome assembly GO:0001675 ISO
    spermatid nucleus differentiation GO:0007289 IBA
    spermatid nucleus differentiation GO:0007289 ISO
    intermediate filament organization GO:0045109 IBA
    intermediate filament organization GO:0045109 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 ISO
    cell projection GO:0042995 ISO
    neuronal cell body GO:0043025 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Agfg1 in sEVs
1
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Agfg1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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