Gene description for Carm1
Gene name coactivator-associated arginine methyltransferase 1
Gene symbol Carm1
Other names/aliases Prmt4
Species Rattus norvegicus
 Database cross references - Carm1
ExoCarta ExoCarta_363026
Vesiclepedia VP_363026
Entrez Gene 363026
UniProt Q4AE70  
 Carm1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Carm1
Molecular Function
    transcription cis-regulatory region binding GO:0000976 ISO
    transcription cis-regulatory region binding GO:0000976 ISS
    transcription coactivator activity GO:0003713 ISO
    transcription coactivator activity GO:0003713 ISS
    protein binding GO:0005515 IPI
    protein methyltransferase activity GO:0008276 ISO
    histone arginine N-methyltransferase activity GO:0008469 ISO
    protein-arginine N-methyltransferase activity GO:0016274 IEA
    protein-arginine N-methyltransferase activity GO:0016274 ISO
    protein-arginine N-methyltransferase activity GO:0016274 ISS
    nuclear receptor coactivator activity GO:0030374 ISO
    nuclear receptor coactivator activity GO:0030374 ISS
    protein-arginine omega-N asymmetric methyltransferase activity GO:0035242 ISO
    protein-arginine omega-N asymmetric methyltransferase activity GO:0035242 ISS
    histone H3R17 methyltransferase activity GO:0035642 ISO
    histone H3R17 methyltransferase activity GO:0035642 ISS
    histone methyltransferase activity GO:0042054 ISO
    histone methyltransferase activity GO:0042054 ISS
    protein homodimerization activity GO:0042803 ISO
    histone H4R3 methyltransferase activity GO:0044020 IEA
    histone H3K37 methyltransferase activity GO:0062122 IEA
    lysine-acetylated histone binding GO:0070577 ISO
    lysine-acetylated histone binding GO:0070577 ISS
    histone H3R2 methyltransferase activity GO:0070611 IBA
    histone H3R2 methyltransferase activity GO:0070611 ISO
    histone H3R2 methyltransferase activity GO:0070611 ISS
    DNA-binding transcription factor binding GO:0140297 ISO
    histone H3R8 methyltransferase activity GO:0140592 IEA
    histone H3K56 methyltransferase activity GO:0140759 IEA
    histone H3R26 methyltransferase activity GO:0140903 ISO
    histone H3R26 methyltransferase activity GO:0140903 ISS
    histone H4K12 methyltransferase activity GO:0140984 IEA
    histone H2AQ104 methyltransferase activity GO:1990259 IEA
Biological Process
    regulation of growth plate cartilage chondrocyte proliferation GO:0003420 ISO
    chromatin organization GO:0006325 IEA
    chromatin remodeling GO:0006338 IBA
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of DNA-templated transcription GO:0006355 ISO
    mRNA processing GO:0006397 IEA
    protein methylation GO:0006479 ISO
    cell population proliferation GO:0008283 ISO
    positive regulation of cell population proliferation GO:0008284 IMP
    positive regulation of cell population proliferation GO:0008284 ISO
    RNA splicing GO:0008380 IEA
    nuclear receptor-mediated steroid hormone signaling pathway GO:0030518 ISO
    estrogen receptor signaling pathway GO:0030520 ISO
    methylation GO:0032259 IEA
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 ISO
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 ISS
    positive regulation of fat cell differentiation GO:0045600 ISO
    positive regulation of fat cell differentiation GO:0045600 ISS
    positive regulation of transcription by RNA polymerase I GO:0045943 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    response to cAMP GO:0051591 IDA
    endochondral bone morphogenesis GO:0060350 ISO
    protein localization to chromatin GO:0071168 ISO
    replication fork reversal GO:0071932 ISO
    replication fork reversal GO:0071932 ISS
    positive regulation of epithelial cell apoptotic process GO:1904037 ISO
    negative regulation of dendrite development GO:2000171 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    chromosome GO:0005694 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    protein-containing complex GO:0032991 ISO
    nuclear replication fork GO:0043596 ISO
    nuclear replication fork GO:0043596 ISS
    RNA polymerase II transcription regulator complex GO:0090575 ISO
 Experiment description of studies that identified Carm1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Carm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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