ExoCarta: Gene summary

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Gene description for Rangap1
Gene name	RAN GTPase activating protein 1
Gene symbol	Rangap1
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Rangap1
ExoCarta	ExoCarta_362965
Entrez Gene	362965
Rangap1 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Rangap1

Molecular Function
	protein binding	GO:0005515	IPI
	GTPase activator activity	GO:0005096	IEA
	Ran GTPase binding	GO:0008536	IDA
	ubiquitin protein ligase binding	GO:0031625	IPI
Biological Process
	cellular response to vasopressin	GO:1904117	IEP
	response to axon injury	GO:0048678	IEP
	signal transduction	GO:0007165	IEA
	cellular response to peptide hormone stimulus	GO:0071375	IDA
	negative regulation of protein export from nucleus	GO:0046826	ISO
	positive regulation of GTPase activity	GO:0043547	IEA
Subcellular Localization
	cytoplasmic periphery of the nuclear pore complex	GO:1990723	IDA
	cytoplasm	GO:0005737	ISO
	nucleus	GO:0005634	IDA
	dendrite	GO:0030425	IDA
	mitotic spindle	GO:0072686	IDA
	perinuclear region of cytoplasm	GO:0048471	ISO
	nuclear membrane	GO:0031965	ISO
	kinetochore	GO:0000776	ISO
	axon part	GO:0033267	IDA
	cytosol	GO:0005829	IDA
	nuclear pore cytoplasmic filaments	GO:0044614	IDA
	nuclear pore	GO:0005643	IDA

Experiment description of studies that identified Rangap1 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting