Gene description for Spns1
Gene name spinster homolog 1 (Drosophila)
Gene symbol Spns1
Other names/aliases RGD1305613
Species Rattus norvegicus
 Database cross references - Spns1
ExoCarta ExoCarta_361648
Vesiclepedia VP_361648
Entrez Gene 361648
UniProt Q2YDU8  
 Spns1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Spns1
Molecular Function
    transmembrane transporter activity GO:0022857 IBA
    transmembrane transporter activity GO:0022857 IEA
Biological Process
    lipid transport GO:0006869 IEA
    phospholipid efflux GO:0033700 IBA
    phospholipid efflux GO:0033700 IEA
    phospholipid efflux GO:0033700 ISO
    regulation of lysosomal lumen pH GO:0035751 IEA
    regulation of lysosomal lumen pH GO:0035751 ISO
    lysophospholipid transport GO:0051977 IBA
    lysophospholipid transport GO:0051977 IEA
    lysophospholipid transport GO:0051977 ISO
    transmembrane transport GO:0055085 IEA
Subcellular Localization
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IEA
    membrane GO:0016020 IBA
 Experiment description of studies that identified Spns1 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Spns1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Spns1 is involved
No pathways found





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