Gene description for Ube2m
Gene name ubiquitin-conjugating enzyme E2M
Gene symbol Ube2m
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ube2m
ExoCarta ExoCarta_361509
Vesiclepedia VP_361509
Entrez Gene 361509
 Ube2m identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Ube2m
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    ATP binding GO:0005524 IEA
    NEDD8 transferase activity GO:0019788 IBA
    NEDD8 transferase activity GO:0019788 ISO
Biological Process
    protein modification process GO:0036211 ISO
    positive regulation of neuron apoptotic process GO:0043525 IMP
    protein neddylation GO:0045116 IBA
    protein neddylation GO:0045116 ISO
    regulation of postsynapse assembly GO:0150052 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Ube2m in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ube2m
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Grm8  
Two-hybrid Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ube2m is involved
No pathways found





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