Gene description for Cul4a
Gene name cullin 4A
Gene symbol Cul4a
Other names/aliases RGD1563853
Species Rattus norvegicus
 Database cross references - Cul4a
ExoCarta ExoCarta_361181
Vesiclepedia VP_361181
Entrez Gene 361181
 Cul4a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Cul4a
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin ligase complex scaffold activity GO:0160072 ISO
Biological Process
    in utero embryonic development GO:0001701 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    DNA damage response GO:0006974 IBA
    DNA damage response GO:0006974 ISO
    spermatogenesis GO:0007283 ISO
    cell population proliferation GO:0008283 ISO
    positive regulation of cell population proliferation GO:0008284 ISO
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 ISO
    hemopoiesis GO:0030097 ISO
    negative regulation of granulocyte differentiation GO:0030853 ISO
    cellular response to UV GO:0034644 ISO
    somatic stem cell population maintenance GO:0035019 ISO
    T cell activation GO:0042110 ISO
    ribosome biogenesis GO:0042254 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    positive regulation of protein catabolic process GO:0045732 ISO
    regulation of protein metabolic process GO:0051246 ISO
    ubiquitin-dependent protein catabolic process via the C-end degron rule pathway GO:0140627 ISO
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
    regulation of DNA damage checkpoint GO:2000001 ISO
    regulation of nucleotide-excision repair GO:2000819 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 IBA
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 ISO
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 ISO
 Experiment description of studies that identified Cul4a in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cul4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cul4a is involved
No pathways found





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