Gene description for Rnasel
Gene name ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
Gene symbol Rnasel
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rnasel
ExoCarta ExoCarta_359726
Vesiclepedia VP_359726
Entrez Gene 359726
 Rnasel identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Rnasel
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    RNA nuclease activity GO:0004540 IBA
    RNA nuclease activity GO:0004540 IDA
    RNA nuclease activity GO:0004540 ISO
    protein kinase activity GO:0004672 IEA
    ATP binding GO:0005524 IEA
    rRNA binding GO:0019843 IDA
    identical protein binding GO:0042802 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
Biological Process
    rRNA processing GO:0006364 IDA
    RNA processing GO:0006396 IBA
    mRNA processing GO:0006397 IEA
    regulation of mRNA stability GO:0043488 ISO
    negative regulation of viral genome replication GO:0045071 IBA
    negative regulation of viral genome replication GO:0045071 ISO
    fat cell differentiation GO:0045444 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of D-glucose import GO:0046326 ISO
    defense response to virus GO:0051607 IBA
Subcellular Localization
    nuclear matrix GO:0016363 IDA
 Experiment description of studies that identified Rnasel in sEVs
1
Experiment ID 92
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Rnasel
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rnasel is involved
No pathways found





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