Gene description for Rasal3
Gene name RAS protein activator like 3
Gene symbol Rasal3
Other names/aliases A430107D22Rik
Species Mus musculus
 Database cross references - Rasal3
ExoCarta ExoCarta_320484
Vesiclepedia VP_320484
Entrez Gene 320484
UniProt Q8C2K5  
 Rasal3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rasal3
Molecular Function
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    negative regulation of Ras protein signal transduction GO:0046580 ISO
    negative regulation of Ras protein signal transduction GO:0046580 ISS
    positive regulation of NK T cell proliferation GO:0051142 ISO
    positive regulation of NK T cell proliferation GO:0051142 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cell cortex GO:0005938 IEA
    cytoplasmic side of membrane GO:0098562 IEA
    cytoplasmic side of membrane GO:0098562 ISO
 Experiment description of studies that identified Rasal3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rasal3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Usp7  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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