 Experiment description of studies that identified Hist4h4 in exosomes |
| 1 |
| Experiment ID |
10 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [MALDI TOF]
|
| PubMed ID |
11390481
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. |
| Authors |
"Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S." |
| Journal name |
JIMMU
|
| Publication year |
2001 |
| Sample |
Dendritic cells |
| Sample name |
D1 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.15-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting |
|
|
| 2 |
| Experiment ID |
210 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23260141
|
| Organism |
Mus musculus |
| Experiment description |
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. |
| Authors |
"Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL." |
| Journal name |
Cell
|
| Publication year |
2012 |
| Sample |
Fibroblasts |
| Sample name |
Normal-Fibroblasts (L cells) |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 3 |
| Experiment ID |
15 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17486113
|
| Organism |
Mus musculus
Homo sapiens |
| Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
| Authors |
"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" |
| Journal name |
NCB
|
| Publication year |
2007 |
| Sample |
Mast cells |
| Sample name |
MC9
Bone marrow-derived mast cells
HMC-1 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.11-1.21 g/mL
|
| Molecules identified in the study |
Protein
mRNA
miRNA |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array |
|
|
| 4 |
| Experiment ID |
264 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25242146
|
| Organism |
Mus musculus |
| Experiment description |
Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells |
| Authors |
"Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino" |
| Journal name |
Molecular Cell
|
| Publication year |
2014 |
| Sample |
Neural stem cells |
| Sample name |
NPCs - Th2 treated |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.20 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 5 |
| Experiment ID |
33 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
Alix|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21136642
|
| Organism |
Mus musculus |
| Experiment description |
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? |
| Authors |
"Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J" |
| Journal name |
PROTEOMICS_CL
|
| Publication year |
2007 |
| Sample |
Oligodendrocytes |
| Sample name |
Oligodendrocytes
Oli-neu |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy |
|
|
