Gene description for Spic
Gene name Spi-C transcription factor (Spi-1/PU.1 related)
Gene symbol Spic
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Spic
ExoCarta ExoCarta_314711
Vesiclepedia VP_314711
Entrez Gene 314711
 Spic identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Spic
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO
Biological Process
    blastocyst development GO:0001824 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    cell differentiation GO:0030154 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
 Experiment description of studies that identified Spic in sEVs
1
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method RT-PCR
Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Spic
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Spic is involved
No pathways found





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