Gene description for HLF
Gene name hepatic leukemia factor
Gene symbol HLF
Other names/aliases -
Species Homo sapiens
 Database cross references - HLF
ExoCarta ExoCarta_3131
Entrez Gene 3131
HGNC 4977
MIM 142385
UniProt Q16534  
 HLF identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for HLF
Molecular Function
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    double-stranded DNA binding GO:0003690 TAS
    DNA binding GO:0003677 TAS
    sequence-specific DNA binding GO:0043565 IEA
Biological Process
    regulation of transcription, DNA-templated GO:0006355 IEA
    multicellular organismal development GO:0007275 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    skeletal muscle cell differentiation GO:0035914 IEA
    rhythmic process GO:0048511 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
 Experiment description of studies that identified HLF in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for HLF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATF4  
Affinity Capture-MS Homo sapiens
2 DDIT3 1649
Affinity Capture-MS Homo sapiens
3 HLF 3131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 BATF  
Affinity Capture-MS Homo sapiens
5 CEBPG  
Affinity Capture-MS Homo sapiens
6 TEF  
Affinity Capture-MS Homo sapiens
7 DBP  
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which HLF is involved
No pathways found





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