Gene description for Tmem106b
Gene name transmembrane protein 106B
Gene symbol Tmem106b
Other names/aliases LRRGT00101
Species Rattus norvegicus
 Database cross references - Tmem106b
ExoCarta ExoCarta_312132
Vesiclepedia VP_312132
Entrez Gene 312132
UniProt Q6AYA5  
 Tmem106b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tmem106b
Molecular Function
    protein binding GO:0005515 IPI
    ATPase binding GO:0051117 ISO
Biological Process
    lysosome organization GO:0007040 IBA
    lysosome organization GO:0007040 ISO
    lysosomal transport GO:0007041 IBA
    lysosomal transport GO:0007041 ISO
    lysosomal lumen acidification GO:0007042 ISO
    biological_process GO:0008150 ND
    lysosome localization GO:0032418 IBA
    lysosome localization GO:0032418 ISO
    lysosome localization GO:0032418 ISS
    dendrite morphogenesis GO:0048813 IBA
    dendrite morphogenesis GO:0048813 ISO
    dendrite morphogenesis GO:0048813 ISS
    positive regulation of hydrolase activity GO:0051345 ISO
    neuron cellular homeostasis GO:0070050 ISO
    positive regulation of dendrite development GO:1900006 ISO
    lysosomal protein catabolic process GO:1905146 ISO
    regulation of lysosome organization GO:1905671 ISO
Subcellular Localization
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISS
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    endosome GO:0005768 ISO
    plasma membrane GO:0005886 IEA
    late endosome membrane GO:0031902 IEA
 Experiment description of studies that identified Tmem106b in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tmem106b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tmem106b is involved
No pathways found





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