ExoCarta: Gene summary

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Gene description for Sppl2a
Gene name	signal peptide peptidase-like 2A
Gene symbol	Sppl2a
Other names/aliases	RGD1563001
Species	Rattus norvegicus
Database cross references - Sppl2a
ExoCarta	ExoCarta_311401
Vesiclepedia	VP_311401
Entrez Gene	311401
Sppl2a identified in sEVs derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Sppl2a

Molecular Function
	aspartic endopeptidase activity, intramembrane cleaving	GO:0042500	IBA
	aspartic endopeptidase activity, intramembrane cleaving	GO:0042500	ISO
	protein homodimerization activity	GO:0042803	ISO
Biological Process
	membrane protein ectodomain proteolysis	GO:0006509	ISO
	membrane protein intracellular domain proteolysis	GO:0031293	ISO
	membrane protein proteolysis	GO:0033619	IBA
	membrane protein proteolysis	GO:0033619	ISO
	regulation of immune response	GO:0050776	ISO
Subcellular Localization
	lysosomal membrane	GO:0005765	IBA
	lysosomal membrane	GO:0005765	ISO
	late endosome	GO:0005770	ISO
	plasma membrane	GO:0005886	IEA
	plasma membrane	GO:0005886	ISO
	membrane	GO:0016020	ISO
	Golgi-associated vesicle membrane	GO:0030660	IBA
	Golgi-associated vesicle membrane	GO:0030660	ISO
	late endosome membrane	GO:0031902	ISO
	intracellular membrane-bounded organelle	GO:0043231	ISO
	lumenal side of endoplasmic reticulum membrane	GO:0098553	IBA
	lumenal side of endoplasmic reticulum membrane	GO:0098553	ISO
	cytoplasmic side of endoplasmic reticulum membrane	GO:0098554	IBA
	cytoplasmic side of endoplasmic reticulum membrane	GO:0098554	ISO

Experiment description of studies that identified Sppl2a in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting