Gene description for Sema5a
Gene name sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Gene symbol Sema5a
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Sema5a
ExoCarta ExoCarta_310207
Entrez Gene 310207
UniProt D3ZTD8  
 Sema5a identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Sema5a
Molecular Function
    semaphorin receptor binding GO:0030215 ISS
    chondroitin sulfate proteoglycan binding GO:0035373 IMP
    heparan sulfate proteoglycan binding GO:0043395 IMP
    axon guidance receptor activity GO:0008046 ISO
    syndecan binding GO:0045545 IPI
Biological Process
    semaphorin-plexin signaling pathway GO:0071526 ISS
    cell chemotaxis GO:0060326 ISS
    blood vessel endothelial cell proliferation involved in sprouting angiogenesis GO:0002043 ISS
    axonal fasciculation GO:0007413 IDA
    branching morphogenesis of an epithelial tube GO:0048754 ISO
    positive regulation of axon extension involved in axon guidance GO:0048842 IMP
    negative regulation of cell adhesion GO:0007162 ISS
    positive regulation of protein kinase B signaling GO:0051897 ISS
    positive regulation of endothelial cell proliferation GO:0001938 ISS
    negative regulation of axon extension involved in axon guidance GO:0048843 IMP
    axon guidance GO:0007411 ISO
    diencephalon development GO:0021536 IMP
    signal clustering GO:1990256 ISS
    positive chemotaxis GO:0050918 ISS
    negative regulation of endothelial cell apoptotic process GO:2000352 ISS
    positive regulation of catenin import into nucleus GO:0035413 ISS
    positive regulation of endothelial cell chemotaxis GO:2001028 ISS
    positive regulation of angiogenesis GO:0045766 ISS
    positive regulation of actin filament depolymerization GO:0030836 ISS
    patterning of blood vessels GO:0001569 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 ISO
 Experiment description of studies that identified Sema5a in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Sema5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sema5a is involved
PathwayEvidenceSource
Other semaphorin interactions IEA Reactome





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