Gene description for Fbxw4
Gene name F-box and WD-40 domain protein 4
Gene symbol Fbxw4
Other names/aliases Dac
Fbw4
SHFM3
SHSF3
dactylin
dactylyn
Species Mus musculus
 Database cross references - Fbxw4
ExoCarta ExoCarta_30838
Vesiclepedia VP_30838
Entrez Gene 30838
UniProt Q9JMJ2  
 Fbxw4 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Fbxw4
Biological Process
    positive regulation of mesenchymal cell proliferation GO:0002053 IMP
    Wnt signaling pathway GO:0016055 IEA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
    embryonic digit morphogenesis GO:0042733 IMP
    cartilage development GO:0051216 IMP
    limb development GO:0060173 IGI
    limb development GO:0060173 IMP
Subcellular Localization
    SCF ubiquitin ligase complex GO:0019005 IBA
 Experiment description of studies that identified Fbxw4 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Fbxw4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Skp1a 21402
Affinity Capture-Western Mus musculus
2 Ercc4  
Co-fractionation Mus musculus
3 Cul1 26965
Affinity Capture-Western Mus musculus
4 Mast1  
Co-fractionation Mus musculus
5 Nanog  
Affinity Capture-Western Mus musculus
6 Rnf20  
Co-fractionation Mus musculus
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