Gene description for Rbx1
Gene name ring-box 1, E3 ubiquitin protein ligase
Gene symbol Rbx1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rbx1
ExoCarta ExoCarta_300084
Vesiclepedia VP_300084
Entrez Gene 300084
 Rbx1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rbx1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IC
    ubiquitin-protein transferase activity GO:0004842 ISO
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin-ubiquitin ligase activity GO:0034450 ISO
    protein-containing complex binding GO:0044877 IPI
    molecular adaptor activity GO:0060090 ISO
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 ISO
    NEDD8 ligase activity GO:0061663 ISO
    cullin family protein binding GO:0097602 IBA
    cullin family protein binding GO:0097602 ISO
Biological Process
    protein polyubiquitination GO:0000209 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    protein monoubiquitination GO:0006513 ISO
    protein monoubiquitination GO:0006513 ISO
    DNA damage response GO:0006974 ISO
    spermatogenesis GO:0007283 ISO
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 ISO
    protein catabolic process GO:0030163 ISO
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    negative regulation of type I interferon production GO:0032480 ISO
    cellular response to oxidative stress GO:0034599 ISO
    cellular response to UV GO:0034644 ISO
    T cell activation GO:0042110 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    protein neddylation GO:0045116 ISO
    positive regulation of protein catabolic process GO:0045732 ISO
    protein K48-linked ubiquitination GO:0070936 ISO
    protein K48-linked ubiquitination GO:0070936 ISO
    cellular response to amino acid stimulus GO:0071230 ISO
    ubiquitin-dependent protein catabolic process via the C-end degron rule pathway GO:0140627 ISO
    positive regulation of protein autoubiquitination GO:1902499 ISO
    negative regulation of response to oxidative stress GO:1902883 ISO
    positive regulation of TORC1 signaling GO:1904263 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    SCF ubiquitin ligase complex GO:0019005 ISO
    VCB complex GO:0030891 IDA
    cullin-RING ubiquitin ligase complex GO:0031461 IBA
    cullin-RING ubiquitin ligase complex GO:0031461 ISO
    Cul2-RING ubiquitin ligase complex GO:0031462 ISO
    Cul3-RING ubiquitin ligase complex GO:0031463 ISO
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 ISO
    Cul4B-RING E3 ubiquitin ligase complex GO:0031465 ISO
    Cul5-RING ubiquitin ligase complex GO:0031466 ISO
    Cul7-RING ubiquitin ligase complex GO:0031467 ISO
 Experiment description of studies that identified Rbx1 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rbx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rbx1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here