Gene description for Clic4
Gene name chloride intracellular channel 4 (mitochondrial)
Gene symbol Clic4
Other names/aliases D0Jmb3
TU-74
mc3s5
mtCLIC
Species Mus musculus
 Database cross references - Clic4
ExoCarta ExoCarta_29876
Vesiclepedia VP_29876
Entrez Gene 29876
UniProt Q9QYB1  
 Clic4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Microglia 16081791    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Clic4
Molecular Function
    chloride channel activity GO:0005254 IBA
    chloride channel activity GO:0005254 ISO
    protein binding GO:0005515 IPI
    oxidoreductase activity GO:0016491 IEA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    angiogenesis GO:0001525 IMP
    endothelial cell morphogenesis GO:0001886 IMP
    chloride transport GO:0006821 IBA
    chloride transport GO:0006821 ISO
    vacuolar acidification GO:0007035 IMP
    fertilization GO:0009566 IMP
    keratinocyte differentiation GO:0030216 IMP
    keratinocyte differentiation GO:0030216 ISO
    negative regulation of cell migration GO:0030336 ISO
    negative regulation of cell migration GO:0030336 ISS
    multicellular organism growth GO:0035264 IMP
    branching morphogenesis of an epithelial tube GO:0048754 IMP
    retina vasculature morphogenesis in camera-type eye GO:0061299 IMP
    cellular response to calcium ion GO:0071277 IEA
    cellular response to calcium ion GO:0071277 ISO
    chloride transmembrane transport GO:1902476 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 IEA
    microvillus GO:0005902 ISO
    cell-cell junction GO:0005911 IEA
    cell-cell junction GO:0005911 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    microtubule cytoskeleton GO:0015630 ISO
    membrane GO:0016020 IBA
    membrane GO:0016020 ISS
    nuclear matrix GO:0016363 IEA
    nuclear matrix GO:0016363 ISO
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    cytoplasmic vesicle membrane GO:0030659 IEA
    chloride channel complex GO:0034707 IEA
    apical part of cell GO:0045177 IEA
    apical part of cell GO:0045177 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Clic4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Clic4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 MAPT  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which Clic4 is involved
No pathways found





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