Gene description for Gsn
Gene name gelsolin
Gene symbol Gsn
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gsn
ExoCarta ExoCarta_296654
Entrez Gene 296654
UniProt Q68FP1  
 Gsn identified in exosomes derived from the following tissue/cell type
Cortical neurones 16446100    
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gsn
Molecular Function
    actin binding GO:0003779 ISO
    myosin II binding GO:0045159 ISO
    protein domain specific binding GO:0019904 ISO
    calcium ion binding GO:0005509 ISO
Biological Process
    regulation of establishment of T cell polarity GO:1903903 ISO
    actin filament severing GO:0051014 ISS
    extracellular matrix disassembly GO:0022617 ISO
    tissue regeneration GO:0042246 IEP
    renal protein absorption GO:0097017 ISO
    barbed-end actin filament capping GO:0051016 IEA
    response to folic acid GO:0051593 IEP
    actin filament reorganization GO:0090527 ISO
    positive regulation of gene expression GO:0010628 ISO
    actin filament polymerization GO:0030041 ISS
    actin polymerization or depolymerization GO:0008154 IDA
    positive regulation of protein processing in phagocytic vesicle GO:1903923 ISO
    amyloid fibril formation GO:1990000 ISO
    phosphatidylinositol-mediated signaling GO:0048015 IMP
    hepatocyte apoptotic process GO:0097284 ISO
    actin nucleation GO:0045010 IEA
    regulation of wound healing, spreading of epidermal cells GO:1903689 ISO
    regulation of plasma membrane raft polarization GO:1903906 ISO
    oligodendrocyte development GO:0014003 IEP
    negative regulation of viral entry into host cell GO:0046597 ISO
    regulation of receptor clustering GO:1903909 ISO
    sequestering of actin monomers GO:0042989 ISO
    actin filament capping GO:0051693 ISO
    cilium morphogenesis GO:0060271 ISS
    apoptotic process GO:0006915 IDA
    wound healing GO:0042060 IEP
    phagocytosis, engulfment GO:0006911 ISO
    regulation of podosome assembly GO:0071801 ISO
    striated muscle atrophy GO:0014891 ISO
    response to ethanol GO:0045471 IEP
    vesicle-mediated transport GO:0016192 ISO
    positive regulation of actin nucleation GO:0051127 ISO
    positive regulation of keratinocyte apoptotic process GO:1902174 ISO
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:2001269 ISO
    aging GO:0007568 IEP
    regulation of cell adhesion GO:0030155 IMP
    cellular response to cadmium ion GO:0071276 IDA
    protein destabilization GO:0031648 ISO
Subcellular Localization
    focal adhesion GO:0005925 ISO
    myelin sheath GO:0043209 IDA
    podosome GO:0002102 ISO
    plasma membrane GO:0005886 ISO
    cortical actin cytoskeleton GO:0030864 ISO
    extracellular region GO:0005576 ISS
    nucleus GO:0005634 ISO
    extracellular space GO:0005615 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    actin cap GO:0030478 ISO
    lamellipodium GO:0030027 ISO
    blood microparticle GO:0072562 ISO
    ruffle GO:0001726 IDA
    actin cytoskeleton GO:0015629 IDA
    cytosol GO:0005829 ISS
    sarcoplasm GO:0016528 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    protein complex GO:0043234 IDA
 Experiment description of studies that identified Gsn in exosomes
1
Experiment ID 30
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
FLOT
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 92
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
3
Experiment ID 93
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
4
Experiment ID 94
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gsn
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gsn is involved
PathwayEvidenceSource
Caspase-mediated cleavage of cytoskeletal proteins IEA Reactome





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