Gene description for Gpi
Gene name glucose-6-phosphate isomerase
Gene symbol Gpi
Other names/aliases Amf
Gpi1
Nlk
Pgi
Phi
Species Rattus norvegicus
 Database cross references - Gpi
ExoCarta ExoCarta_292804
Vesiclepedia VP_292804
Entrez Gene 292804
UniProt Q6P6V0  
 Gpi identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gpi
Molecular Function
    glucose-6-phosphate isomerase activity GO:0004347 IBA
    glucose-6-phosphate isomerase activity GO:0004347 IDA
    glucose-6-phosphate isomerase activity GO:0004347 IEA
    glucose-6-phosphate isomerase activity GO:0004347 ISO
    cytokine activity GO:0005125 IEA
    growth factor activity GO:0008083 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    monosaccharide binding GO:0048029 IBA
    monosaccharide binding GO:0048029 IPI
    carbohydrate derivative binding GO:0097367 IEA
Biological Process
    in utero embryonic development GO:0001701 ISO
    mesoderm formation GO:0001707 ISO
    positive regulation of immunoglobulin production GO:0002639 ISO
    fructose 6-phosphate metabolic process GO:0006002 ISO
    gluconeogenesis GO:0006094 IBA
    gluconeogenesis GO:0006094 IEA
    gluconeogenesis GO:0006094 ISO
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IEA
    glycolytic process GO:0006096 ISO
    signal transduction GO:0007165 IEA
    learning or memory GO:0007611 IEP
    positive regulation of endothelial cell migration GO:0010595 ISO
    response to estradiol GO:0032355 IEP
    response to progesterone GO:0032570 IEP
    response to testosterone GO:0033574 IEP
    erythrocyte homeostasis GO:0034101 ISO
    response to immobilization stress GO:0035902 IEP
    response to muscle stretch GO:0035994 IEP
    glucose homeostasis GO:0042593 ISO
    negative regulation of apoptotic process GO:0043066 IMP
    response to cadmium ion GO:0046686 IEP
    glucose 6-phosphate metabolic process GO:0051156 IBA
    glucose 6-phosphate metabolic process GO:0051156 IDA
    glucose 6-phosphate metabolic process GO:0051156 ISO
    GDP-mannose biosynthetic process from glucose GO:0141199 ISO
Subcellular Localization
    extracellular space GO:0005615 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    ciliary membrane GO:0060170 ISO
 Experiment description of studies that identified Gpi in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gpi
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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