Gene description for Sf3b3
Gene name splicing factor 3b, subunit 3
Gene symbol Sf3b3
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Sf3b3
ExoCarta ExoCarta_292019
Vesiclepedia VP_292019
Entrez Gene 292019
 Sf3b3 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Sf3b3
Molecular Function
    U2 snRNA binding GO:0030620 IBA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    biological_process GO:0008150 ND
    negative regulation of protein catabolic process GO:0042177 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    U2-type spliceosomal complex GO:0005684 ISO
    U2 snRNP GO:0005686 IBA
    U12-type spliceosomal complex GO:0005689 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    U2-type precatalytic spliceosome GO:0071005 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Sf3b3 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sf3b3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sf3b3 is involved
No pathways found





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