ExoCarta: Gene summary

Credits | Contact

Gene description for Capns1
Gene name	calpain, small subunit 1
Gene symbol	Capns1
Other names/aliases	Capn4
Species	Rattus norvegicus
Database cross references - Capns1
ExoCarta	ExoCarta_29156
Vesiclepedia	VP_29156
Entrez Gene	29156
UniProt	Q64537
Capns1 identified in sEVs derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Capns1

Molecular Function
	calcium-dependent cysteine-type endopeptidase activity	GO:0004198	IDA
	calcium-dependent cysteine-type endopeptidase activity	GO:0004198	IEA
	calcium-dependent cysteine-type endopeptidase activity	GO:0004198	ISO
	calcium ion binding	GO:0005509	IEA
	calcium ion binding	GO:0005509	TAS
	protein binding	GO:0005515	IPI
	protein-containing complex binding	GO:0044877	IPI
Biological Process
	proteolysis	GO:0006508	ISO
	protein catabolic process	GO:0030163	IMP
Subcellular Localization
	cytoplasm	GO:0005737	IEA
	cytoplasm	GO:0005737	ISO
	cytosol	GO:0005829	IEA
	cytosol	GO:0005829	ISO
	plasma membrane	GO:0005886	IEA
	endomembrane system	GO:0012505	IEA
	membrane	GO:0016020	IEA
	membrane	GO:0016020	ISO
	calpain complex	GO:0110158	IBA
	calpain complex	GO:0110158	IEA
	calpain complex	GO:0110158	ISO

Experiment description of studies that identified Capns1 in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting