Gene description for Atp8b1
Gene name ATPase, aminophospholipid transporter, class I, type 8B, member 1
Gene symbol Atp8b1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Atp8b1
ExoCarta ExoCarta_291555
Vesiclepedia VP_291555
Entrez Gene 291555
 Atp8b1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Atp8b1
Molecular Function
    magnesium ion binding GO:0000287 IEA
    lipid transporter activity GO:0005319 ISO
    ATP binding GO:0005524 IEA
    aminophospholipid flippase activity GO:0015247 IEA
    aminophospholipid flippase activity GO:0015247 ISO
    ATP hydrolysis activity GO:0016887 IEA
    phosphatidylcholine floppase activity GO:0090554 IEA
    phosphatidylserine floppase activity GO:0090556 IEA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 IBA
    phosphatidylcholine flippase activity GO:0140345 IEA
    phosphatidylcholine flippase activity GO:0140345 ISO
    phosphatidylserine flippase activity GO:0140346 IMP
    cardiolipin binding GO:1901612 IEA
    cardiolipin binding GO:1901612 ISO
Biological Process
    xenobiotic transmembrane transport GO:0006855 IEA
    xenobiotic transmembrane transport GO:0006855 ISO
    Golgi organization GO:0007030 IBA
    sensory perception of sound GO:0007605 IEA
    sensory perception of sound GO:0007605 ISO
    biological_process GO:0008150 ND
    bile acid metabolic process GO:0008206 IEA
    bile acid metabolic process GO:0008206 ISO
    organic anion transport GO:0015711 ISO
    bile acid and bile salt transport GO:0015721 IEA
    bile acid and bile salt transport GO:0015721 ISO
    aminophospholipid transport GO:0015917 ISO
    vestibulocochlear nerve formation GO:0021650 IEA
    vestibulocochlear nerve formation GO:0021650 ISO
    regulation of microvillus assembly GO:0032534 IEA
    regulation of microvillus assembly GO:0032534 ISO
    apical protein localization GO:0045176 IEA
    apical protein localization GO:0045176 ISO
    phospholipid translocation GO:0045332 IBA
    phospholipid translocation GO:0045332 ISO
    negative regulation of DNA-templated transcription GO:0045892 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    inner ear receptor cell development GO:0060119 IEA
    inner ear receptor cell development GO:0060119 ISO
    aminophospholipid translocation GO:0140331 IEA
    regulation of plasma membrane organization GO:1903729 IEA
    regulation of plasma membrane organization GO:1903729 ISO
    regulation of chloride transport GO:2001225 IEA
    regulation of chloride transport GO:2001225 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
    brush border membrane GO:0031526 IDA
    stereocilium GO:0032420 IEA
    stereocilium GO:0032420 ISO
    phospholipid-translocating ATPase complex GO:1990531 IEA
    phospholipid-translocating ATPase complex GO:1990531 ISO
    phospholipid-translocating ATPase complex GO:1990531 ISS
 Experiment description of studies that identified Atp8b1 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atp8b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atp8b1 is involved
PathwayEvidenceSource
Ion channel transport IEA Reactome
Ion transport by P-type ATPases IEA Reactome
Transport of small molecules IEA Reactome





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