Gene description for Actr2
Gene name ARP2 actin-related protein 2 homolog (yeast)
Gene symbol Actr2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Actr2
ExoCarta ExoCarta_289820
Vesiclepedia VP_289820
Entrez Gene 289820
UniProt Q5M7U6  
 Actr2 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Actr2
Molecular Function
    actin binding GO:0003779 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    cytoskeletal protein binding GO:0008092 IDA
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 ISO
Biological Process
    establishment or maintenance of cell polarity GO:0007163 IEA
    establishment or maintenance of cell polarity GO:0007163 ISO
    associative learning GO:0008306 IEP
    asymmetric cell division GO:0008356 IEA
    asymmetric cell division GO:0008356 ISO
    positive regulation of lamellipodium assembly GO:0010592 ISO
    meiotic chromosome movement towards spindle pole GO:0016344 IEA
    meiotic chromosome movement towards spindle pole GO:0016344 ISO
    cytosolic transport GO:0016482 IEA
    cytosolic transport GO:0016482 ISO
    actin cytoskeleton organization GO:0030036 IEA
    actin cytoskeleton organization GO:0030036 ISO
    meiotic cytokinesis GO:0033206 IEA
    meiotic cytokinesis GO:0033206 ISO
    Arp2/3 complex-mediated actin nucleation GO:0034314 IBA
    Arp2/3 complex-mediated actin nucleation GO:0034314 ISO
    Arp2/3 complex-mediated actin nucleation GO:0034314 ISS
    response to immobilization stress GO:0035902 IEP
    cellular response to trichostatin A GO:0035984 IEP
    response to ethanol GO:0045471 IEP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    meiotic cell cycle GO:0051321 ISO
    spindle localization GO:0051653 IEA
    spindle localization GO:0051653 ISO
    cilium assembly GO:0060271 ISO
    positive regulation of dendritic spine morphogenesis GO:0061003 IMP
    cellular response to type II interferon GO:0071346 IEA
    cellular response to type II interferon GO:0071346 ISO
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISO
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISS
    regulation of double-strand break repair via nonhomologous end joining GO:2001032 ISO
    regulation of double-strand break repair via nonhomologous end joining GO:2001032 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytoskeleton GO:0005856 IEA
    Arp2/3 protein complex GO:0005885 IBA
    Arp2/3 protein complex GO:0005885 ISO
    Arp2/3 protein complex GO:0005885 ISS
    cell cortex GO:0005938 IBA
    cell cortex GO:0005938 ISO
    lamellipodium GO:0030027 IDA
    actin cap GO:0030478 IEA
    actin cap GO:0030478 ISO
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISS
    podosome core GO:0061825 IDA
    postsynapse GO:0098794 EXP
    postsynapse GO:0098794 IDA
 Experiment description of studies that identified Actr2 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Actr2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snca  
Affinity Capture-MS Rattus norvegicus
2 Gja1  
Affinity Capture-MS Rattus norvegicus
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