Gene description for Adss
Gene name adenylosuccinate synthase
Gene symbol Adss
Other names/aliases Adss2
Species Rattus norvegicus
 Database cross references - Adss
ExoCarta ExoCarta_289276
Vesiclepedia VP_289276
Entrez Gene 289276
 Adss identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Adss
Molecular Function
    magnesium ion binding GO:0000287 IEA
    adenylosuccinate synthase activity GO:0004019 IBA
    adenylosuccinate synthase activity GO:0004019 IDA
    adenylosuccinate synthase activity GO:0004019 ISO
    GTP binding GO:0005525 IEA
Biological Process
    purine nucleotide biosynthetic process GO:0006164 ISO
    AMP biosynthetic process GO:0006167 ISO
    AMP biosynthetic process GO:0006167 ISO
    aspartate metabolic process GO:0006531 IDA
    response to purine-containing compound GO:0014074 IEP
    'de novo' AMP biosynthetic process GO:0044208 IBA
    'de novo' AMP biosynthetic process GO:0044208 IEA
    'de novo' AMP biosynthetic process GO:0044208 ISO
    IMP metabolic process GO:0046040 IBA
    IMP metabolic process GO:0046040 IDA
    response to ammonium ion GO:0060359 IEP
    cellular response to electrical stimulus GO:0071257 IEP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Adss in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Adss
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Adss is involved
No pathways found





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